apc anti human cd73 antibody  (Miltenyi Biotec)

Journal: bioRxiv

Article Title: Donor-matched iPSC model reveals context-dependent T2D genetic signals in fibro-adipogenic progenitors

doi: 10.64898/2026.02.04.702388

Figure Lengend Snippet: A – A heatmap comparing reference genotyping data from the FUSION study to low-pass genotyping data generated as part of QC metrics for this study. The fraction match indicates the fraction of reads that aligned from the low pass WGS data to the reference SNP Chip. B – Histogram of FAP marker gene expression based on FACS assessment of 30 different lines. The X-axis indicates the percentage of cells per line expressing NT5E. The average across all cell lines was 74% of cells expressing the FAP marker gene. C – Sankey plot representing the insulin-stimulated glucose uptake assay. D – Comparison of normalized luminescence from all 30 donors (with replicates) in basal, basal with a small dose of insulin, high-insulin, and high-insulin with a small additional dose of insulin. Pink lines indicate the average luminescence value for that environmental condition. A paired Student’s t-test was used to assess statistical significance. E – An interval plot comparing donor metadata to luminescence levels in basal conditions. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM. F – An interval plot comparing donor metadata to luminescence levels in high-insulin conditions. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM. G – An interval plot comparing donor metadata to luminescence levels in basal conditions with an additional small dose of insulin. Data represented as mean ± SEM. H – An interval plot comparing donor metadata to luminescence levels in high-insulin conditions with an additional small dose of insulin. Red indicates a negative correlation with glucose uptake. Data represented as mean ± SEM.

Article Snippet: Cells are then centrifuged down, media is aspirated, and the positive sample is stained with antibodies for Tra-1-60 (iPSC marker) (Miltenyi Biotec Cat# 130-122-921, RRID:AB_2801969) and NT5E (FAP marker) (Miltenyi Biotec Cat# 130-111-913, RRID:AB_2784275) for 10 minutes.

Techniques: Generated, Marker, Gene Expression, Expressing, Comparison

Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A , B Western blot analysis for CD141, CD31, VE-cadherin, LYVE1, FGFR2, ETV2, TNAP, αSMA, Transgelin, c-MET, and GAPDH in VMSCs and ADSCs and quantitative densitometry analysis using the NIH ImageJ program. GAPDH was used as a loading control. Values are mean ± SD of 3 independent experiments (* P < 0.05, ** P < 0.01, and *** P < 0.001, n.s.: not significant, Student’s t test). Quantification of HGF ( C ), uPA ( D ), VEGF ( E ), and Angiogenin ( F ) in conditioned medium of VMSCs and ADSCs by ELISA. Values are mean ± SD of 3 independent experiments (* P < 0.05, ** P < 0.01, Student’s t test). G Tube formation assay of VMSC, ADSC, and a combination of VMSC and ADSC in a 2:1 ratio in the presence or absence of 500 pg/ml TNF-α. Scale bar: 500 μm. H Quantitative analysis of tube based on number of meshes, number of master segments, total master segments length, and number of isolated segments using NIH ImageJ angiogenesis analyzer. Values are mean ± SD of 4 independent experiments (*** P < 0.001; V (-TNF-α) VS. V (+TNF-α), $$$ P < 0.001; 2:1 (-TNF-α) VS. 2:1 (+TNF-α), # P < 0.05, ## P < 0.01; n.s.: not significant, One-way ANOVA test followed by Tukey’s multiple comparison test. V: VMSC, A: ADSC.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Isolation, Comparison

Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A Western blot of CD141, CD31, αSMA, Transgelin, and GAPDH expression in VMSCs and ADSCs after treatment of 10 ng/ml TNF-α for 24 or 48 h. Quantitative densitometry analysis of CD141 ( B ) CD31 ( C ) αSMA ( D ) and Transgelin ( E ) using NIH ImageJ program. GAPDH was used as a loading control. Each value is expressed as a fold change relative to VMSCs without TNF-α treatment (24 h). Values are mean ± SD of 3 independent experiments. Statistical analyses were conducted exclusively against the respective control groups. (* P < 0.05, *** P < 0.001, Student’s t test). F Gelatin zymography to detect activity of pro-MMP-9, pro-MMP-2, and active-MMP-2 in conditioned medium of VMSCs and ADSCs. Quantitative densitometry analysis of pro-MMP-9 ( G ) pro-MMP-2 ( H ) and active-MMP-2 ( I ). Values are mean ± SD of 3 independent experiments. Total protein lysate (μg) of each group was used as loading control. Statistical analyses were conducted exclusively against the respective control groups. (* P < 0.05, ** P < 0.01, and *** P < 0.001, Student’s t test). Quantification of HGF ( J ) and VEGF ( K ) in conditioned medium in VMSCs and ADSCs by ELISA. Values are mean ± SD of 3 independent experiments. Total protein lysate (μg) of each group was used as loading control. Statistical analyses were conducted exclusively against the respective control groups (** P < 0.01, *** P < 0.001, Student’s t test). L The tube formation assay with VMSC, ADSC and a combination of VMSC (V) and ADSC (A) in a 2:1 ratio was performed after priming of TNF-α for 24 h. Scale bar: 500 μm. M Quantitative analysis of tube architecture was performed based on the number of meshes, the number of master segments, total master segments length, and the number of isolated segments using NIH ImageJ angiogenesis analyzer. Values are mean ± SD of 4 independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001; VMSC (V) TNF-α (-) VS. V TNF-α (+), $ P < 0.05, $$ P < 0.01; 2:1 TNF-α (-) VS. 2:1 TNF-α (+), # P < 0.05, ## P < 0.01, One-way ANOVA test followed by Tukey’s multiple comparison test). V: VMSC, A: ADSC.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Western Blot, Expressing, Control, Zymography, Activity Assay, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Isolation, Comparison

Journal: NPJ Regenerative Medicine

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia

doi: 10.1038/s41536-026-00458-x

Figure Lengend Snippet: A Representative fluorescence images of Human/Mouse CD31 + blood vessels at day 28. Scale bar: 100 μm. B , C Quantification of Human/Mouse CD31 + blood vessels analyzed by NIH image J analyzer. Values are mean ± SD of 5 independent experiments (*** P < 0.001, Student’s t test). D Fluorescence images of Human/Mouse Transgelin + blood vessels at day 28. Scale bar: 100 μm. E , F Quantification of Human/Mouse Transgelin + blood vessels analyzed by NIH ImageJ analyzer. Values are mean ± SD of 5 independent experiments (*** P < 0.001, Student’s t test). G Representative fluorescence images of Human CD31 + blood vessels at day 28 post transplantation. Scale bar: 100 μm. Orange scale bar in high mag. image: 50 μm. H Quantification of diameter distribution of human CD31 + blood vessels by NIH ImageJ analyzer. I Double fluorescence staining for Human CD31 and Human/Mouse CD141. White arrow head: colocalization of CD31 and CD141. Scale bar: 50 μm. J The detection of human nuclear antigen (HN) in vessels in dual cell-transplanted muscle. Yellow arrow head indicates human antigen. Scale bar: 20 μm. N = 8/group.

Article Snippet: The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec).

Techniques: Fluorescence, Transplantation Assay, Staining